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Fig. 6. APC2 co-localizes with actin throughout denticle development. Embryos, anterior left. (A,B) APC2 (red), actin (green). Late condensations. (C-H) Stills, movie of embryo expressing UAS-APC2-GFP driven throughout the epidermis using 69B-GAL4. Times in hours:minutes. (C,D) APC2-GFP localizes apically in cells of presumptive denticle belts (brackets). (E-H) APC2-GFP localizes to posterior margin (E, arrows), and condensations focus and brighten (F-H). Colored arrowheads – condensations merging. (I-L) APC2. Wild type (I) and dsh⁷⁵ (J,K) or arm^XM19 (L) maternal and zygotic mutants. APC2 (red, single channel in L) localizes to denticle primordia even when they arise at the cell apex (arrowheads, J-L). (I,J) Coracle (green; outlining cells). (K) F-actin (phalloidin; green). Scale bars, 10 µm.

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