JCS02755 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. ARAP3-deficient cells do not form protrusive lamellipodia. (A) 32D6 cells were seeded onto glass coverslips, starved for 16 hours, stimulated for 5 minutes with PDGF or its vehicle (as indicated), and fixed. Cells were stained for F-actin. An arrow points to a 32D6 cell carrying an actin-rich protrusion. The photographs are representative examples taken from two independent experiments. (B) 32D6 cells were treated as in (A) and stained for paxillin. Short arrows point to typical clustered focal adhesions. Bars, 20 mm.

• Supplemental Figure 2 -

  Fig. S2. ARAP3-deficient cells do not polarise well. 32D6 cells were seeded onto coverslips and left to grow until confluent. Monolayers were scratched with a sterile pipette tip and left to migrate to close the ‘wound’. (A) Cells were fixed 4.5 hours after scratching and stained for F-actin. (B) Cells were fixed 3 hours after scratching and stained for a-tubulin. The shown data is representative from two independent experiments. A thin broken line indicates the orientation of the ‘wound’ in the photographs. Bar, 20 mm.