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Figure 2


Fig. 2. ARAP3-deficient cells do not polarise well. NA1 or 52B3 cells were seeded onto coverslips and left to grow until confluent. Confluent monolayers were scratched with a sterile pipette tip and left to migrate to close the `wound'. (A) Cells were fixed 4.5 hours after scratching, and stained to visualise F-actin. (B) Cells were fixed 3 hours after scratching and stained to visualise {alpha}-tubulin. Data similar to the photographs shown (A,B) were obtained in two further experiments. A thin broken white line indicates the orientation of the `wound' in each photograph. Bar, 20 µm. (C) Cells were fixed 0.5, 3 and 9 hours after scratching and stained for giantin with a nuclear counterstain. Cells in which the Golgi apparatus lay within the third of the cell facing the `wound' were counted as polarised. Black bars, NA1 control cells; unfilled bars, 52B3; grey bars, 32D6 ARAP3 RNAi cells. The graph integrates data from three independent experiments.





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