spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. ARAP3-deficient cells have increased activities of RhoA. (A) NA1 and 52B3 cells were transiently transfected with a Myc-tagged ROCK construct. After 16 hours of serum starvation, cells were or were not stimulated with PDGF or its vehicle for 1-15 minutes and lysed. ROCK protein was immunoprecipitated using an anti-Myc antibody and subsequently used for an in vitro kinase assay. The graph represents data pooled (means ± s.d.) from three independent experiments, which were all done in duplicate. The PDGF-stimulated increase in ROCK activity in the 52B3 cells is statistically significant (*P=0.05 in a paired Student's t-test). (B) NA1 and 52B3 cells were seeded into tissue culture dishes, serum starved for 16 hours and lysed straight away or after stimulation with PDGF or its vehicle for 1-10 minutes. Lysates were subjected to western blotting and probed with an anti phospho-MYPT antibody and subsequently with a control antibody (ß-COP) as a loading control. The shown blot is a representative example from three independent experiments.





Right arrow Return to article