JCS02756 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Accumulation of GFP-Akt-PH (PIP₃) at the formed phagocytic cup of a neutrophilic HL60 cell. This is the complete data set from which Fig. 1b was taken.

• Movie 2 -

  Movie 2. The 3D reconstructed distribution of GFP-Akt-PH [PtdIns(3,4,5)P₃] at a near-complete phagosome is shown. This forms part of Fig. 2.

• Movie 3 -

  Movie 3. A typical time series showing the distribution of formation of DiIC₁₆(3) in a neutrophil undergoing phagocytosis. A single image from this data set is shown as Fig. 3c.

• Movie 4 -

  Movie 4. The asynchronous accumulation of GFP-Akt-PH [PtdIns(3,4,5)P₃] at two formed phagocytic cups (around particles Z1 and Z2) of a neutrophilic HL60 cell. This is the complete time course from which the images in Fig. 6a were taken.

• Movie 5 -

  Movie 5. The simultaneous monitoring of cytosolic free Ca²⁺ changes (fluo4) and PIP₃ accumulation (GFP-Akt-PH) at the formed phagocytic cup of a neutrophilic HL60 cell is shown. This is the complete data set from which Fig. 8b was extracted.

• Movie 6 -

  Movie 6. The accumulation of GFP-Akt-PH (PIP₃) at the firm contact point (indentation rather than phagocytic cup) of a neutrophilic HL60 cell treated with cytochalasin B. This is the complete data set from which Fig. 8d was taken.