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Figure 1


Fig. 1. PtdIns(3,4,5)P3 accumulation at the phagocytic cup. (a) Phase-contrast (i and iii) and confocal fluorescent images (ii and iv) of neutrophilic HL60 cells expressing GFP-Akt-PH during phagocytosis. Images i and ii are approximately 30 seconds earlier than images iii and iv, respectively. (b) A series of confocal images of a single phagocytic cup formed around a zymosan particle at the time intervals indicated, showing that GFP-Akt-PH accumulation follows cup formation. The complete data set for this can be seen in Movie 1 of supplementary material. (c) Time course of the intensity of GFP-Akt-PH at the phagocytic cup and at the membrane locations either side (±3 µm). (d) Distribution of GFP-Akt-PH intensity at the membrane in the region of the phagocytic cup, with the boundaries of the phagocytic cup marked. All the images are representative of at least ten similar examples. Bars, 3 µm.





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