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Figure 4


Fig. 4. Mobility of PtdIns(3,4,5)P3 in neutrophilic cells. The effects of localised photobleaching on GFP-Akt-PH. In each data set, the first image shows the distribution of GFP-Akt-PH before laser photobleaching, the middle image is the zone of localised bleaching marked by the rectangle and the last image shows the effect 30 seconds after bleaching in a resting neutrophilic HL60 cell (a), a neutrophilic HL60 cell undergoing phagocytosis (b) and a neutrophilic HL60 cell with a completed phagosome (c). These data are typical of at least three similar experiments in each case. Bars, 2 µm.





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