spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Cholesterol depletion disrupts PtdIns(3,4,5)P3 accumulation. (a) Distribution of filipin staining in fixed neutrophils before and after treatment with methyl-ß-cyclodextrin. (b) Quantification of the reduction in `peripheral' or membrane staining in neutrophils before and after treatment with methyl-ß-cyclodextrin. The data are shown as the percentage of filipin staining at the cell periphery. (c) Images of a neutrophilic HL60 cell treated with methyl-ß-cyclodextrin demonstrating the failure of GFP-Akt-PH to accumulate at phagocytic cups. The cell was stained with DiIC16(3) (red) and GFP-Akt-PH (green) with the position of two zymosan particles indicated. This cell was typical of at least ten others. Bars, 4 µm.





Right arrow Return to article