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Figure 6


Fig. 6. Assembly of IFN-{gamma}-induced MVP into intact vault particles. (A) Cell lysates obtained from the indicated cell lines incubated for 24 hours in the absence or presence of IFN-{gamma} (250 IU/ml) were fractionated as described in Materials and Methods. Total cell lysates (L) are opposed to a 100,000 g particle (P) and a high-speed supernatant fraction (S). Similar amounts of protein were separated by SDS-PAGE, immunoblotted and analyzed for presence of MVP. One of three experiments leading to comparable results is shown. (B) KB-3-1 cells were grown on chamber slides, treated with IFN-{gamma} (250 IU/ml) for the indicated times, fixed and immunostained for MVP (green). Nuclei were counterstained with DAPI (blue). Bar, 5 µm.





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