spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. DN STAT3 blocks insulin-induced PKC{delta} activation. Keratinocytes were either uninfected (–), or infected with DN STAT3 adenovirus. Following infection (24 hours), cells were untreated, or stimulated with 10–7 M insulin (Ins) for the designated times (5 or 30 minutes). (A) PKC{delta} or (B) STAT3 were immunoprecipitated from protein extracts using specific anti-PKC{delta} and anti-STAT3 antibodies. PKC{delta} and STAT3 immunoprecipitates were analyzed for PKC activity using an in vitro kinase assay as described in Materials and Methods. Each bar represents the mean ± s.d. of three determinations in three separate experiments. Values are expressed as picomoles of ATP per dish per minute. Highly significant differences were found (P<0.0001) for both IP PKC{delta} and STAT3 compared with levels in the respective controls at 5 minutes.





Right arrow Return to article