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Figure 1


Fig. 1. Overexpression of JAM-A induces HUVEC migration. (A) Western blot of protein lysate (50 µg) from mock- and JAM-A-transfected HUVECs. The lower blot shows the upper blot after reprobing using anti-{alpha}-tubulin to ensure equal loading. (B) Densitometric analysis of (A) indicates JAM-A overexpression is about three times that of endogenous JAM-A expression. (C) A wound-induced migration assay on vitronectin matrix was performed using mock- and JAM-A-transfected cells in the absence of serum or growth factors. The extent of cell migration into the wounded area was photographed under phase-contrast microscopy at 0 hour and after 24 hours. Magnification, x100. Data shown are representative of three separate experiments.





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