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Figure 2


Fig. 2. JAM-A-induced HUVEC migration is vitronectin specific. (A) A haptotactic transwell motility assay using mock- and JAM-A-transfected cells was performed on inserts pre-coated with vitronectin. Images are representative of the migration of JAM-A-overexpressing cells in three separate experiments performed in triplicate. Magnification, x200. (B) A haptotactic transwell motility assay using mock- and JAM-A-transfected cells was performed on inserts pre-coated with indicated ECM proteins. (C) Data collected in (B) represented as fold increase over mock. A two- to fivefold increase in migration of JAM-A-overexpressing cells on vitronectin was routinely observed, and was dependent upon the passage number of transfected cells.





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