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Fig. 3. JAM-A does not bind to vitronectin. (A) HUVECs stably transfected with JAM-A or mock transfected were allowed to adhere to various concentrations of vitronectin as indicated for 1 hour. Bound cells were quantified as described in the Materials and Methods. (B) ELISA standard curve using soluble JAM-A as indicated. (C) In vitro binding assay of soluble JAM-A to immobilized vitronectin. Data shown are expressed as mean ± s.e.m. of three separate experiments.