spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. The actin-binding ability of SWAP-70 through the C-terminal region is crucial for membrane ruffling induced by EGF. (A) COS7 cells expressing GFP or GFP–SWAP-70(1-564) were treated with (+) or without (–) EGF (100 ng/ml) for 5 minutes and stained with Phalloidin to detect F-actin. Localization of SWAP-70 (green) and F-actin (red) was observed under the confocal microscope. Stacked images are indicated by `S', and dorsal planes of focus by `D'. Membrane ruffles are indicated by arrowheads. Bar, 20 µm. (B) COS7 cells expressing GFP or GFP–SWAP-70(1-564) were treated with EGF (100 ng/ml) for 5 minutes and stained with Phalloidin to detect F-actin. Ruffling formation was quantified by scores defined in the Materials and Methods.





Right arrow Return to article