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Fig. 6. Large-scale translocation of bead adhesions in response to prolonged force. The position of individual surface-bound magnetic beads coated with RGD peptide or K20 antibody was recorded in the absence or presence of sustained (2 minutes) application of force (130 pN) in a centripetal direction, opposite to that in which the beads normally move on the surface of the cell. (A) Measured displacements of RGD-beads in the absence of force (
; –F) and in the presence of force (+ F) without (
) or with Y27 (
) or BDM (
). Displacements of K20-beads in the absence of force (
; –F) or presence of force (+F) without (
) or with soluble RGD peptide (sRGD, 
). (B) Representative X-Y plot of positions of a representative RGD-bead before (), during (black line), and after (
) 2 minute application of force (130 pN). The bead was initially displaced toward the magnet, but was then actively retracted against the magnetic gradient and towards the nucleus during the course of force application (arrow indicates direction of applied force).
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