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Figure 2


Fig. 2. (A) Partial nuclear division in slk19{Delta} cells occurs prior to initiation of anaphase. cdc20{Delta} GAL-CDC20 (US3398) and cdc20{Delta} slk19{Delta} GAL-CDC20 (US3399) cells were arrested in G1 using {alpha} factor and released into YEPD at 24°C. At the end of 4 hours, the cells were collected and analyzed for the state of nuclear (DAPI) division (top panel and bottom graph; for each time point, 150 cells were counted). In a parallel experiment, G1 synchronized cdc20{Delta} (US3568) and cdc20{Delta} slk19{Delta} (US3579) cells containing SCC1-cmyc18 were released into YEPD at 24°C. Samples were analyzed for the levels of Scc1, Clb2 and tubulin, and DNA content. *Denotes cleaved Scc1 (middle panel). To check whether partial nuclear division occurs in another kinetochore-component-defective mutant, cdc20{Delta} ndc10-1 GAL-CDC20 (US4708) cells were subjected to identical experimental regime as described above (bottom panel). (B) Spindles in slk19{Delta} cells. Samples taken at 240 minutes from both US3398 and US3399 cells as described in (A) were stained using anti-Tub4 antibodies.





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