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Figure 3


Fig. 3. Bipolar attachment in slk19{Delta} cells. WT (US3437) and slk19{Delta} (US3502) strains carrying TetO/GFP-TetR constructs were arrested in G1 using {alpha} factor and released into YEPD at 24°C. Samples withdrawn every 15 minutes were analyzed for state of nuclear division, centromeric markers (for each time point, 150 cells were counted) and DNA content. The bottom panels show the state of GFP-marked centromeric markers at 90', 105' and 120' time points in both WT and slk19{Delta} cells.





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