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Fig. 5. (A) Partial nuclear division in slk19 ${Delta}$ results from the force exerted by the spindle. G1 synchronized cdc20 ${Delta}$ GAL-CDC20 (US3398) and cdc20 ${Delta}$ slk19 ${Delta}$ GAL-CDC20 (US3399) strains were released into YEPD containing nocodazole (15 µg/ml). Panel a shows cdc20 ${Delta}$ and panel b cdc20 ${Delta}$ slk19 ${Delta}$ cells. Top row shows cells at 240 minutes after release from ${alpha}$ factor into YEPD. Middle row shows cells at 240 minutes after release from ${alpha}$ factor into YEPD containing nocodazole. Bottom row shows cells that were first arrested in G2-M with short spindle (240 minutes) and then treated with nocodazole for 1.5 hours. (Panel c) Co-localization of Ndc10 with Slk19 in cdc20 ${Delta}$ GAL-CDC20 cells (US4194) arrested in G2-M by Cdc20 depletion in glucose medium. (B) Spindle length distribution in cdc20 ${Delta}$ and cdc20 ${Delta}$ slk19 ${Delta}$ cells. cdc20 ${Delta}$ GAL-CDC20 (US3398) and cdc20 ${Delta}$ slk19 ${Delta}$ GAL-CDC20 (US3399) cells were arrested in G1 using ${alpha}$ factor and released into YEPD at 24°C. At the end of 4 hours, cells were stained with anti-tubulin antibodies. The spindle length was determined using Metamorph software (a total of 270 cells were counted). (C) Greater variation in distance between SPBs in slk19 ${Delta}$ cells. WT (US3786) and slk19 ${Delta}$ (US4164) strains expressing Spc42-GFP integrated at the TRP1 locus were immobilized onto gelatin coated slides and immunofluorescent signals were observed every 5 minutes. The distance between the Spc42-GFP spots is plotted versus time.

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