spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. (A) Onset of Scc1 cleavage remains unaffected in the absence of Slk19. cdc20{Delta} GAL-CDC20 (US3910) and cdc20 slk19{Delta} GAL-CDC20 (US3579) strains were arrested in G1 at 24°C using {alpha} factor and then released into raffinose medium to deplete Cdc20. After 240 minutes, cells were filtered and resuspended in galactose medium pre-equilibrated at 16°C to induce Cdc20 synthesis. Scc1 cleavage was monitored by western blot analysis and DNA content was measured by FACS in samples collected at various time points. (B) Chromatin Immunoprecipitation. Cross linked chromatin from WT SCC1-cmyc18 SLK19-HA6 (US3779, left panel) and slk19{Delta} SCC1-cmyc18 (US3516, right panel) was divided into two portions. One portion was used as input DNA. The other half was immunoprecipitated with anti-HA or anti-cmyc beads. The input DNA and coimmunoprecipitated samples were analyzed with PCR (24 cycles) using pairs of primers (see Materials and methods) corresponding to the following loci; CEN3, CEN16 and MET2.





Right arrow Return to article