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Figure 9


Fig. 9. (A) Slk19-GFP is localized to the kinetochores in the absence of Scc1 function. Scc1-73 (US4077) and wild-type (US2337) cells containing SLK19-GFP were arrested in G1 using {alpha} factor and released at 37°C. Samples were taken every 15 minutes and analyzed for state of nuclear division and GFP signal. (B) Reduced cleavage of Slk19-HA6 in the absence of Scc1 function. Scc1-73 SLK19-HA6 cells (US4530) were arrested G1 using {alpha} factor and released into either 24°C or 37°C medium. Samples were taken every 15 minutes and analyzed by western blotting.





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