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Figure 5


Fig. 5. Inhibition of Myosin-IE by free Mg2+. The rate of ADP dissociation from Myosin-IE is dependent on the Mg2+ concentration. The kobs for the displacement of 30 µM ADP from 0.25 µM E698 by 500 µM MgATP is plotted over the range 0 to 20 mM free-Mg2+ concentration. The data were fitted to a hyperbola and indicate a Kd of 0.99±0.3 mM.





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