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Figure 3


Fig. 3. Expression of ephrin-B1, EphB2, EphB6 and the cytoplasmic adaptor protein Dishevelled in cortical and medullary tubule cells. (A) RT-PCR amplification of mRNA prepared from cultured cortical and medullary tubule cells shows that the cells in culture retain ephrin-B1, EphB2 and EphB6 expression patterns similar to those in the regions of the kidney from which the cells are derived (see Fig. 1B,C). (B) Densitometric quantification of ephrin-B1, EphB2 and EphB6 amplification products from cortical and medullary tubule cells. Data from three independent experiments, normalized to the levels of the GAPDH amplification products, are shown as mean ± s.d. Comparisons were performed using unpaired Student's t-test. The levels of the EphB2 and EphB6 amplification products are significantly higher in medullary than in cortical tubule cells (P=0.0001 and P=0.009, respectively), whereas the expression level of ephrin-B1 is not significantly different between the two (P=0.367).





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