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Figure 1


Fig. 1. The subcellular distribution of PtdIns4KII{alpha} in HT1080 cells. (A) Live cells expressing GFP-PtdIns4KII{alpha} were imaged by confocal laser-scanning microscopy. (B) Detail of plasma membrane (PM), small cytoplasmic vesicles (SV) and tubules (T). (C) Detail of the juxta-nuclear region showing multivesicular body (MVB)-like structures (MV). (D) Endogenous PtdIns4KII{alpha} in fixed HT1080 cells and (E) detail. (F) Cells co-immunostained for endogenous PtdIns4KII{alpha} and lamp-1. Staining for (G) GFP-PtdIns4KII{alpha} and lamp-1, (H) CD63 and (I) LBPA. Arrows in G,H indicate colocalisation of GFP-PtdIns4KII{alpha} and marker in punctate structures. Arrows in I indicate the localisation of GFP-PtdIns4KII{alpha} to the boundary membrane of MVB-like structures. All zoom boxes are 20 µm square. Bars, 10 µm.





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