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Figure 3


Fig. 3. DEX decreases Runx2 serine phosphorylation. Primary dermal fibroblasts were transduced with Runx2 retrovirus or left unmodified for controls and cultured in osteogenic media with and without 10 nM DEX. (A) Runx2 protein levels were examined at 7 days post-transduction by western blotting of whole-cell lysates with a polyclonal antibody against Runx2. GAPDH was used as a loading control. (B) Quantification of Runx2 band intensities [mean ± s.e.m., n=9; ANOVA: P<0.05; **different from unmodified and DEX-only controls (P<0.05)]. (C) Runx2 phosphoserine levels were assessed by immunoprecipitation of whole-cell lysates with an antibody against Runx2 and western blotting with antibodies against Runx2 and phosphoserine. (D) Quantification of Runx2 phosphoserine band intensities [mean ± s.e.m., n=9; ANOVA: P<0.05; {ddagger}different from Runx2+DEX (P< 0.05)].





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