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Figure 5


Fig. 5. Mutation of Ser125 decreases Runx2 serine phosphorylation. Primary dermal fibroblasts were transduced with Runx2-WT or Runx2-125Gly retrovirus and cultured in osteogenic media with (+) and without (–) 10 nM DEX. (A) Runx2 phosphoserine levels were assessed by immunoprecipitation of whole-cell lysates with an antibody against Runx2 and western blotting with antibodies against Runx2 and phosphoserine (pSerine). (B) Quantification of normalized Runx2 phosphoserine band intensities [mean ± s.e.m., n=3; ANOVA: P<0.05; {ddagger}different from Runx2+DEX, ¥different from 125Gly-DEX, ##different from 125Gly+DEX (P< 0.05); representative data from three separate experiments in triplicate].





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