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Figure 7


Fig. 7. Constitutive phosphorylation of Runx2-Ser125 inhibits osteoblastic differentiation. Cells were transduced with Runx2-WT, Runx2-125Gly or Runx2-125Glu retrovirus and cultured in osteogenic media with (+) and without (–) 10 nM DEX. (A) ALP activity was examined by a biochemical assay at 7 days post-transduction [mean ± s.e.m., n=6; ANOVA: P<1E-11; **different from unmodified and DEX-only controls, {dagger}different from Runx2-DEX, §different from 125Glu-DEX and 125Glu+DEX, £different from 125Glu-DEX only (P< 0.05)]. (B) Mineralized matrix deposition was assessed by von Kossa staining for phosphate-positive regions and (C) quantified by image analysis at 14 days post-transduction [mean ± s.e.m., n=6; ANOVA: P<1E-11, **different from unmodified and DEX-only controls, {dagger}different from Runx2-DEX, ¥different from 125Gly-DEX, §different from 125Glu-DEX and 125Glu+Dex, £different from 125Glu-DEX only (P< 0.05)]. Bar, 2 cm.





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