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Fig. 1. Dermal fibroblasts were plated onto collagen-coated glass coverslips at a concentration of 125 cells/mm² in FM for 3-6 hours at 37°C. Migration experiments were performed in FM in the presence or absence of ß-AR agonist (10 nM-100 µM). The migration of each single cell was monitored over a 1-hour period. The speed and distance traveled, at a concentration of 1 µM ß-AR agonist, are represented graphically in A and B, respectively. The ß-AR-mediated dose-dependent increase in distance traveled is represented graphically in C (ß-AR agonist 10 nM-100 µM). The data are representative of three independent experiments with three different fibroblast strains (n=50). Values plotted are mean ± s.e.m. ^*P<0.01 between ß-AR agonist and controls. Cells were starved of growth factors in DMEM for 16 hours. 1-2x10⁷ cells were either left un-treated or treated with 1 µM ß-AR agonist in DMEM for 10 minutes at 37°C. After treatment, cell lysates were prepared and the EGFR was immunoprecipitated. EGFR antibody-associated proteins were electrophoresed on two separate 10% polyacrylamide gels at the same time and transferred to membranes. Membranes were immunoblotted with either an EGFR antibody (EGFR WB) or an anti-phosphotyrosine antibody (PY WB; D). Gels were aligned to allow correct identification of the EGFR protein. The data shown are representative of three independent experiments from three separate cell strains. Three blots from three separate experiments were scanned for EGFR tyrosine phosphorylation and densitometry was performed using a gel plotting macro in NIH Image 1.62. Data was averaged, statistically analyzed and represented graphically (Fig. 1E). Values plotted are mean ± s.e.m. (n=3). ^*P<0.01 between ß-AR agonist and control.

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