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Figure 5


Fig. 5. 5x104 dermal fibroblasts were plated per well in FM in a 12-well plate in triplicate and either untreated (A) or pre-treated with 50 µM sp-cAMP for 30 minutes (B, sp-cAMP alone, +, medium dashed line) or 50 µM rp-cAMP for 30 minutes (C, rp-cAMP alone, +, medium dashed line) prior to the addition of FM alone (control, {circ}, solid line in A-C) or FM containing 1 µM ß-AR agonist alone ({square}, short dashed line in A-C), or ß-AR agonist and sp-cAMP (x, medium dashed line in B) or rp-cAMP (x, long dashed line in C). Cells were harvested and counted on days 2, 4, 6, 8. The data are representative of three independent experiments with three different fibroblast strains. Values plotted are mean ± s.e.m. *P<0.01 between ß-AR agonist and controls.





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