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Figure 2


Fig. 2. LPA inhibits the motility of mitochondria in CV-1 cells through RhoA. (A,C,E) The first frame of the image sequence recorded, and (B,D,F) the plotted tracks of individual mitochondria in the same cells. (A,B) Control cells; (C,D) cells were treated with 5 µM LPA for 5 minutes; (E,F) cells were transfected with plasmid pcDNA3-EGFP-RhoA(Q63L). See also Movie 3 in supplementary material. Bar, 10 µm. (G) Quantification of mitochondrial motility in control cells, cells treated with 5 µM LPA or cells expressing EGFP-RhoA(Q63L) (RhoA-QL). Values are the mean percentage of movements exceeding 0.2 µm/second from all movements ± s.e.m.; P<0.05; n = number of cells and, in brackets, number of organelle movements.





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