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Figure 3


Fig. 3. Effects of a constitutively active mutant of mDia1 on the cytoskeleton of CV-1 cells. Cells were transfected with plasmids encoding EGFP-tagged mDia-{Delta}N3. After fixation in 4% formaldehyde with 1% Triton X-100, cells were stained with TRITC-phalloidin to visualize F-actin (A) or with antibodies against {alpha}-tubulin and TRITC-labeled secondary antibodies (C), and with antibodies against Glu-tubulin and FITC-labeled secondary antibodies to visualize stable microtubules (B,D). Transfected cells were found before fixation by EGFP fluorescence (shown by arrows). Bar, 10 µm.





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