spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. A constitutively active mDia1 mutant, but not other proteins promoting actin polymerization, inhibits the motility of mitochondria. CV-1 cells were transfected with plasmids encoding EGFP-mDia-{Delta}N3, EGFP-Rac1(Q61L), EGFP-Cdc42(Q61L) or EGFP-WA, and the movement of fluorescently labeled mitochondria was recorded by time-lapse video microscopy. The first frames of the image sequences, showing different mitochondria distribution in cells expressing EGFP-mDia-{Delta}N3 (A), and EGFP-Rac1(Q61L) (B) (see Movies 4 and 5 in supplementary material). (D) The cell expressing EGFP-WA (shown with arrow) with increased level of F-actin visualized by TRITC-phalloidin staining. Bars, 10 µm. (C) Quantification of mitochondrial motility in cells expressing EGFP-mDia-{Delta}N (mDia-{Delta}N), EGFP-WA (WA), EGFP-Rac1(Q61L) (Rac1-QL) or EGFP-Cdc42(Q61L) (Cdc-QL). Values are the mean percentage of movements exceeding 0.2 µm/second from all movements ± s.e.m.; P<0.05; n = number of cells and, in brackets, number of organelle movements.





Right arrow Return to article