|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. The constitutively active mutant of diaphanous induces mitochondrial anchoring in Drosophila BG2-C2 cells. BG2-C2 cells were transfected with a plasmid encoding EGFP-C-Dia. Cells were either fixed for cytoskeleton staining (A-F) or incubated with 100 nM Texas Red Mitotracker for the analysis of mitochondrial motility (G-I). (A,D,G) Fluorescent images that show the transfected cells. (C) Cells were stained with anti-tubulin antibody and TRITC-labeled secondary antibody. Microtubules in transfected cells form the characteristic rosette-like pattern. (F) Cells were stained with TRITC-phalloidin; the higher level of F-actin could be seen in transfected cells. (B,E,H) Phase-contrast images of cells shown in A,D,G, and (I) the first frame in the video sequence (Movie 6 in supplementary material) showing the distribution of elongated mitochondria in the transfected cell shown in G. Bars, 10 µm.
|
