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Fig. 8. Disruption of F-actin by latrunculin B increases the motility of mitochondria. The movements of fluorescently labeled mitochondria in CV-1 (A) and BG2-C2 (B) cells were recorded using time-lapse video microscopy before or after incubation with either 0.2 µM latrunculin B (LB) for 20 minutes (A) or with 10 µM latrunculin B for 1 hour (B) and analyzed, as described in the Materials and Methods. For depletion of diaphanous by RNAi, BG2-C2 cells were incubated for 7 days with dsRNA, as in Fig. 7. For the analysis of mitochondrial motility in BG2-C2 cells transfected with EGFP-C-Dia (GFP-C-Dia), they were incubated with 100 nM Texas Red Mitotracker for 40 minutes, and mitochondrial movements were recorded by time-lapse video microscopy using red fluorescence filter set (see also Movie 7 in supplementary material). Values are the mean percentage of fast mitochondria movements ± s.e.m.; P<0.05; n, number of cells and, in brackets, number of organelle movements.
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