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Fig. 3. Thapsigargin-releasable Ca²⁺ stores are decreased and capacitive Ca²⁺ influx is increased in DD keratinocytes. Normal and DD keratinocytes were cultured and loaded with Fura-2 as detailed in Fig. 1. Cells initially were superfused with solution containing: 138 mM NaCl, 2.7 mM KCl, 1.5 mM KH₂PO₄, 1.0 mM Na₂HPO₄, 0.0 mM CaCl₂, 0.05 mM EGTA, 10 mM glucose, pH 7.4, 286 mOsm. After equilibration, 500 nM thapsigargin was applied (black arrows). Although the resulting ER Ca²⁺ release substantially increased intracellular Ca²⁺ in normal keratinocytes, minimal Ca²⁺ release was noted in DD keratinocytes, consistent with depletion of the SERCA-dependent ER Ca²⁺ stores (Table 2). After recovery, the extracellular Ca²⁺ was increased to 0.06 mM (white arrows), initiating capacitive Ca²⁺ entry. Both normal and DD keratinocytes displayed robust capacitive Ca²⁺ entry; however, the capacitive Ca²⁺ entry was proportionately larger in DD keratinocytes (Table 2).

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