Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells
J Cell Sci Renvoisé et al.
119: 680
JCS02782 Supplementary Material
Files in this Data Supplement:
Supplemental Figure 1
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Fig. S1. Presence
of transiently expressed N86 and SMNex2B in the nucleoli (arrow heads) of COS cells, as
judged by DNA-staining with DAPI. Bars, 3 mm.
Supplemental Figure 2
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Fig. S2. Distribution of mutant SMNDex7
(A), SMN472D5 (B) and SMNTudor (C) observed under
transient transfection of immortalized SMA type I fibroblast cells expressing
low levels of endogenous SMN. Immunolocalization of coilin in untransfected (D)
and FP-SMN transfected (E) SMA cells. Immunolocalization of TMG in SMA cells
transfected with FP-SMN (F). Inserts show FP-SMN (left), coilin or TMG (middle)
and merge (right). Bars, 3mm.
Supplemental Figure 3
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Fig. S3. Immunolocalization of endogenous SMN and
localization of the indicated constructs transiently overexpressed in HeLa
cells analysed by fluorescence microscopy. Bar, 3mm.
