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Figure 4


Fig. 4. Analysis of protein domains required for nuclear localization of SMN. (A) The proportion of fluorescence in the nucleus relative to the total fluorescence contained in the entire stack of z-sections is presented to visualize the distribution of ten cells for each construct. (B) The 14.3.3{gamma} protein localized to the cytoplasm. (C) 14.3.3{gamma} fused to the SMN ex2B region remained in the cytoplasm, whereas (D) fusion to the SMN Tudor domain led to accumulation of the protein in the nucleus. Bars, 3 µm.





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