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Figure 2


Fig. 2. Western blot analyses of Cx43 and Cx31 in heart and brain lysates. Lane 1: Cx43+/+; lane 2: Cx43+/31; lane 3: Cx4331/31. Cx43 protein was not detected in Cx4331/31 animals (lane 3). Cx31 was not found in the Cx43+/+ heart and brain (lane 1), whereas it was expressed in Cx43+/31 mice and there was nearly double the amount in Cx4331/31 animals (lane 2 and lane 3). Loading of equal amounts of homogenates was verified by re-immunostaining with monoclonal anti-tubulin.





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