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Figure 3


Fig. 3. Immunofluorescence analysis of Cx43 and Cx31 in heart. Cryosections of Cx43+/+ (A,D), Cx43+/31 (B,E) and Cx4331/31 (C,F) hearts at P0 were immunolabeled with anti-Cx43 (A-C, red), anti-Cx31 (D-F, green) and counterstained with propidium iodide (shown as blue nuclear staining). Cx43 was expressed in Cx43+/+ and Cx43+/31 ventricles, but not in Cx4331/31 ventricles. Cx31 was not detected in Cx43+/+ hearts, but in Cx43+/31 and Cx4331/31 ventricles. Bars, 20 µm.





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