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Figure 2


Fig. 2. Par-1 is required to establish blastoderm polarity. (A,B) Stage 15 embryos stained for Par-1 protein (red). Embryos were injected with (A) buffer or (B) with par-1 dsRNA. (C-P) Embryos were injected prior to cellularization with buffer (C,E,G,I,K,M,O) or par-1 dsRNA (D,F,H,J,L,N,P) and stained with antibodies near the end of cellularization. Blastoderm nuclei are shown in blue in some panels and all panels are oriented apical side towards the top. (C,D) Blastoderm stained with anti-Par-1 (red) and anti-E-cadherin (green). (E,F) Anti-phosphotyrosine (green) staining. (G,H) Anti-Patj staining (green) is expanded along the lateral domains of the par-1(RNAi) blastoderm. (I,J) Anti-Crb staining (green) in the apical domain. (K,L) Anti-Dlg staining (green) is present along the lateral membranes. (M,N) Anti-Delta staining (green) is reduced basolaterally by par-1(RNAi). (O,P) Anti-Tau (green) stains the apical cytoplasm capping the nuclei and extends down past the nuclei.





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