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Figure 3


Fig. 3. Par-1 is required for gastrula ectoderm cell polarity. Embryos injected at the syncitial stage with buffer (A,C,E) or par-1 dsRNA (B,D,F) are shown at stage 9. (A,B) Anti-E-cadherin (red) shows expanded localization within ectoderm, mesoderm and neuroblasts in par-1(RNAi) embryos. (C-F) Embryos triple-labeled for Patj (cyan), Dlg (green) and E-cadherin (red). (C,D) Patj staining is not significantly expanded along lateral membranes in par-1(RNAi). (E,F) Dlg staining is similar in control and par-1(RNAi) embryos.





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