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Figure 2


Fig. 2. Wound inflammatory cytokine mRNA and protein levels are modulated by MK-434 treatment and castration. (A-D) Incisional day-2-wound sections were analysed immunohistochemically and tissue mRNA and protein extracts were analysed by qPCR and immunoblotting, respectively (n=6-7 per treatment group). Day 6 wound sections were analysed immunohistochemically. Arrows indicate immunoreactive cells. Numbers of IL-6-immunopositive cells, overall protein levels and gene expression were reduced in day-2-wounds of castrated animals and those treated with MK-434 (gene expression in castrated animals only). Numbers of TNF-{alpha}-immunoreactive cells, levels of TNF-{alpha} protein and mRNA were also reduced in day-2-wounds of castrated animals. Numbers of TGF-ß1-immunopositive cells and TGF-ß1 protein levels were increased in day-2-wounds of castrated rats; also increased were numbers of TGF-ß1-immunopositive cells in day-6-wounds of castrated animals and of those treated with MK-434. Numbers in D represent relative levels of protein based on optical density readings, normalised to the ß-actin signal. Bars, 20 µm. Data represent mean ± s.d. *P<0.05; **P<0.01.





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