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Figure 5


Fig. 5. Smads are regulated by androgens and castration. Day-2-wound sections were analysed immunohistochemically, wound protein samples by western blotting, and tissue and macrophage-derived mRNA samples by qPCR. (A-B) Numbers of Smad3- and Smad4-immunopositive cells and protein levels of phosphorylated (activated) Smad3 (pS3) were unaffected in wounds of castrated animals and those treated with MK-434. Smad7-positive cell numbers were significantly increased in day-2-wounds of castrated animals and those treated with MK-434. (C) Castration of animals reduced Smad4 and Smad7 mRNA levels in wounds. (D) Treatment of peritoneal macrophages with testosterone or DHT for 18 hours significantly increased the levels of Smad3 mRNA, whereas exposure to DHT but not testosterone increased mean Smad4 mRNA levels and significantly increased Smad7 mRNA levels. Arrows indicate immunostained cells; dashed lines surround blood vessels. Bars, 50 µm. Data represent mean ± s.d. *P<0.05; **P<0.01. Con, control; T7 and T8, 10-7 M and 10-8 M testosterone, respectively; D7 and D8, 10-7 M and 10-8 M DHT, respectively.





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