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Figure 5


Fig. 5. Effect of Ins(1,4,5)P3R type 1 knock-down on testosterone-induced Ca2+ oscillations. (A,B) Cells transiently transfected with Ins(1,4,5)P3R type 1 siRNA (InsP3R1), showed a significant reduction (~80%) of the immunosignal. (C) In Ins(1,4,5)P3R type 1-siRNA transfected cells two types of Ca2+ response to testosterone were observed. The magnitude of the transient was reduced by 68% compared with untransfected cells, or abolished. (D) Control trace in cells not expressing the Ins(1,4,5)P3R siRNA.





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