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Figure 4


Fig. 4. Membrane phenotype of megakaryocytic cells. HPCs grown in serum-free medium in the presence of 100 ng/ml TPO alone (Control), or in combination with 10 µM PD98059 or 50 nM rapamycin were analyzed throughout the culture (from day 0 up to day 12) by flow cytometry using PE- or FITC-conjugated mAbs. The percentages of CD34+, CD41+, CD61+, CD42b+ and CD62+ cells are shown as the mean ± s.e.m. of three independent experiments.





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