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Fig. 6. Cellular localization of cyclin D1 and cyclin D3. Differentiating MKs grown for 3, 6 and 10 days either in the absence (Control) or in presence of PD98059 or rapamycin were cytocentrifuged, fixed and labeled with anti-cyclin D1 (A) or anti-cyclin D3 (B) and then with FITC-conjugated anti-mouse IgGs. Immunofluorescent cells were detected using an optical microscope equipped for immunofluorescence. Original magnifications: A, 630x; B, 400x.