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Fig. 1. Microtubule bunching during Zinnia tracheary element differentiation. (A) Isolated mesophyll cell before addition of inductive hormones. (B) A tracheary element 72 hours post-induction, with cell wall thickenings visible under bright-field optics. (C) Bundles of cortical microtubules labelled by anti-tubulin immunofluorescence. (D) The same cell with coincident cellulose-rich secondary thickenings labelled with Calcofluor White. Bar, 10 µm.