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Figure 1


Fig. 1. The organisation of yolk-sac-like structures in D13 human EBs. (A,B) Phase-contrast image of day 13 EB where (B) is a higher magnification of the boxed area within (A) to demonstrate the appearance of vessels (arrows). (C) PECAM1 and ENG RNA expression detected by RT-PCR at different stages of EB differentiation. (D-H) Staining of day 13 EBs with (D) anti-PECAM1, (E) anti-AFP antibodies, (F) DAPI to highlight nuclei, (G,H) both anti-PECAM1 and anti-AFP antibodies together with DAPI to distinguish the two cell layers. (H) is a higher magnification of the boxed area within (G).





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