spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Sec8 overexpression favors OL morphological differentiation. (A-D) OL progenitors were infected overnight with adenoviruses encoding (A) control EGFP (EGFP-AV) or (B-D) FLAG-tagged Sec8 (Sec8-AV), allowed to differentiate for 3 days, fixed, labeled with anti-MBP- (red) and anti-FLAG- (green) Abs and analyzed by epifluorescence microscopy. OLs were assigned to three morphological categories: (B) low, (C) medium or (D) high complexity. Cell bodies were overexposed in order to observe protein localization in the processes. Bars, 10 µm; px, pixels. (E) Mature MBP-positive cells were plotted as percentage of the total infected cells. (F) The number of OLs in each morphological category is shown as a percentage of the total MBP-positive OLs (500 cells/condition, n=6 from three independent cultures). (G) The areas of 100 randomly chosen MBP-positive cells per condition were measured and plotted. Only infected cells were analyzed. (E-G) White bars, control EGFP-AV; black bars, Sec8-AV infected.





Right arrow Return to article