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Figure 3


Fig. 3. Sec8 overexpression increases the levels of a subset of myelin proteins. OL progenitors were infected with Sec8-AV and either (A) control EGFP-AV or (C) control null-AV and allowed to differentiate for 3 days in defined medium. Cell lysates were analyzed by immunoblot for (A) Sec8, Sec6, OSP/Claudin11, CASK (10 µg protein/lane); MAG, CNP, MBP, actin (2 µg protein/lane) and (B), and data are presented as percentage of control levels. (C) Cell lysates were analyzed for Sec8, MBP, CNP and actin (10 µg protein/lane). n=5 from three independent cultures, * indicates statistically significant differences.





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