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Figure 4


Fig. 4. siRNA-mediated interference with Sec8 expression decreases OL morphological differentiation. OL progenitors were transfected with (A) fluorescently tagged siRNAs (siGloCipB, red) and (B-E) Sec8 or control siRNAs. Arrows in A indicate a siGlo-positive perinuclear punctum. Cells were allowed to differentiate for 2 days and analyzed by (A,C) fluorescence microscopy for MBP and Hoechst or (B) immunoblot for Sec8, Sec6, MBP and actin (n=8 from four independent cultures). (D) The number of mature MBP-positive cells was plotted as a percentage of the total Hoechst-labeled nuclei (5000 cells/condition, n=11, four independent cultures). (E) Morphological complexity of CNP-positive OLs was analyzed for 900 cells/condition (n=6, two independent cultures). Bars, 10 µm.





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